Non-Magnetic Shell Coating of Magnetic Nanoparticles as Key Factor for Cytotoxicity, NUST MISIS Reports
Non-Magnetic Shell Coating of Magnetic Nanoparticles as Key Factor for Cytotoxicity, NUST MISIS Reports
MOSCOW, Aug. 31, 2021 /PRNewswire/ -- Russian scientists have found that coating magnetic nanoparticles with a non-magnetic silica shell coating significantly decreased the viability of cancer cells in a low frequency alternating magnetic field. The coating increases nanoparticles stability, preventing aggregation in endosomes and keeping them as effective magneto-mechanical actuators in a low-frequency alternating magnetic field. The study was published in Colloids and Surfaces B: Biointerfaces.
莫斯科，2021年8月31日美通社/--俄羅斯科學家發現，在磁性納米顆粒上塗覆一層非磁性硅殼塗層，可顯著降低癌細胞在低頻交變磁場中的存活能力。這種塗層提高了納米顆粒的穩定性，防止了內體的聚集，並使它們在低頻交變磁場中保持為有效的磁機械致動器。這項研究發表在膠體和表面B：生物界面.
Biocompatible magnetic nanomaterials have been intensively studied for various applications in biomedicine. They can be remotely controlled over by an external magnetic field, which makes it possible to specifically affect target molecules on the molecular level.
生物相容性磁性納米材料在生物醫學中的各種應用已經得到了廣泛的研究。它們可以被外部磁場遠程控制，這使得在分子水平上具體影響目標分子成為可能。
Magnetic nanoparticles cytotoxicity depends on acting magnetic field parameters, the most significant of which are magnetic field amplitude, frequency, and the duration of action. In a low frequency alternating magnetic field, they rotate, causing mechanical damage to cells.
磁性納米顆粒的細胞毒性依賴於作用磁場參數，其中最顯著的是磁場幅度、頻率和作用時間。在低頻交變磁場中，它們旋轉，對細胞造成機械損傷。
Scientists from NUST MISIS, M.V. Lomonosov Moscow State University, V. Serbsky National Medical Research Center for Psychiatry and Narcology, Siberian State Medical University, National Research Tomsk Polytechnic University, Skoltech, D.I. Mendeleev University of Chemical Technology of Russia, Helmholtz Institute for Pharmaceutical Research Saarland and N.I Pirogov Russian National Research Medical University have found that a non-magnetic shell coat significantly increases the cytotoxicity of magnetic nanoparticles. Two types of iron oxide nanoparticles with the same magnetic core with and without silica shells were synthesized. Nanoparticles with silica shells significantly decreased the viability of human prostate cancer cells in a low frequency alternating magnetic field according to the cytotoxicity test, unlike uncoated nanoparticles.
來自NUST MISIS、M.V.Lomonosov莫斯科國立大學、V.Serbsky國家精神病學和腫瘤學研究中心、西伯利亞國立醫科大學、託木斯克國立理工大學、Skoltech、D.I.門捷列夫化學技術大學、薩爾蘭Helmholtz藥物研究所和N.I.Pirogov俄羅斯國立研究醫科大學的科學家發現，非磁性外殼塗層顯著增加了磁性納米顆粒的細胞毒性。合成了兩種具有相同磁芯的氧化鐵納米粒子，分別帶有和不帶有二氧化硅殼層。根據細胞毒性測試，與未包覆的納米顆粒不同，帶有二氧化硅外殼的納米顆粒在低頻交變磁場中顯著降低了人前列腺癌細胞的活力。
The study has shown that cell death results from the intracellular membrane integrity failure, and the calcium ions concentration increase with the subsequent necrosis. Transmission electron microscopy and dynamic light scattering images showed that uncoated nanoparticles are etched by acidic media in the endosome and form aggregates. As a result, they encounter high endosomal macromolecular viscosity and become unable to rotate efficiently.
研究表明，細胞死亡是細胞內膜完整性破壞的結果，細胞內鈣離子濃度隨隨後的壞死而增加。透射電子顯微鏡和動態光散射圖像顯示，未包覆的納米顆粒被酸性介質蝕刻在內涵體中並形成聚集體。因此，它們會遇到高內質體大分子粘度，並變得不能有效地旋轉。
The scientists assume that effective rotation of nanoparticles causes cell death in a low frequency alternating magnetic field. In turn, silica shell coating increases nanoparticles stability, preventing aggregation in endosomes.
科學家們假設納米顆粒的有效旋轉會導致細胞在低頻交變磁場中死亡。反過來，硅殼塗層增加了納米顆粒的穩定性，防止了內吞體內的聚集。
"Our fundings have both theoretical and practical value. We discovered that the non-magnetic phase increases the colloidal stability of nanoparticles, thus being the key to their effective magneto-mechanical actuation. This is important for the fundamental understanding of the mechanism of magneto-mechanical actuation and what the structural features of nanoparticles should be in order to maximize their cytotoxicity. On the other hand, we have shown that our nanoparticles work, they do cause cell death. The next step would be testing their effectiveness in vivo," noted Artyom Ilyasov, NUST MISIS Biomedical Nanomaterials Laboratory.
他説：“我們的基金既有理論價值，又有實用價值。 我們發現非磁性相增加了納米粒子的膠體穩定性，因此是其有效磁力驅動的關鍵。這對於從根本上理解磁-機械驅動機制以及納米顆粒的結構特徵以最大限度地發揮其細胞毒性是非常重要的。另一方面，我們已經證明了我們的納米粒子是有效的，它們確實會導致細胞死亡。下一步將是在活體內測試它們的有效性。阿爾泰姆·伊利亞索夫首頁--期刊主要分類--期刊細介紹--期刊題錄與文摘--期刊詳細文摘內容
Link to the source: https://en.misis.ru/university/news/science/2021-08/7493/
來源鏈接：https://en.misis.ru/university/news/science/2021-08/7493/
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SOURCE The National University of Science and Technology MISiS
來源：國立科技大學MISIS
譯文內容由第三人軟體翻譯。